Not sterile
Not soaking for the same amounts of time
Not incubating for the same amounts of time
Not spreading evenly
Not having enough time to do more trials
Counting - different people counted
Not THE MOST ACCURATE COUNTING EVER
Wednesday, May 30, 2007
Title of Our Lab Report
Hi Guys!
What do you think of this title?:
"Effectiveness of Mouthwashes in the Inhibition of the Growth of E. coli and Oral Flora"
- Sarah
What do you think of this title?:
"Effectiveness of Mouthwashes in the Inhibition of the Growth of E. coli and Oral Flora"
- Sarah
SWEET INFO
MOUTH (1:10,000)
water - 330 colonies
tom's - 721 colonies
listerine - 2 colonies
scope - 45 colonies
E COLI (1:10,000)
water - 4.65 cm diameter avg + dense little colonies
tom's - 3.3 cm diamter + dense little colonies
listerine - 4 colonies
scope - no plate!
ML < EL < MS < MW < MT < ET < EW
ML similar to EL
MS noticeably more than ML, EL
MT, MW significantly more than ML, EL
MT, MW look abou tthe same area coverage-wise, but MT has more colonies (2 ppl counting)
EW, ET very different from rest: large colony (lawn) in center w/ small, dense colonies on outside
ES plate broke, so we plated it again 5/30
5/29 plated plates:
990 uL mouth/e coli to 10 mL of nutrient broth -> 1: 100. Repeat for 1:10,000
End up with 1 mL of liquid
divide into 4 tubes, 200 uL to each
add 200 uL of liquid (mouthwash) to each tube
wait... 10 min...
plate w/ 100 uL each
wait 1 day.
yeeehawww
water - 330 colonies
tom's - 721 colonies
listerine - 2 colonies
scope - 45 colonies
E COLI (1:10,000)
water - 4.65 cm diameter avg + dense little colonies
tom's - 3.3 cm diamter + dense little colonies
listerine - 4 colonies
scope - no plate!
ML < EL < MS < MW < MT < ET < EW
ML similar to EL
MS noticeably more than ML, EL
MT, MW significantly more than ML, EL
MT, MW look abou tthe same area coverage-wise, but MT has more colonies (2 ppl counting)
EW, ET very different from rest: large colony (lawn) in center w/ small, dense colonies on outside
ES plate broke, so we plated it again 5/30
5/29 plated plates:
990 uL mouth/e coli to 10 mL of nutrient broth -> 1: 100. Repeat for 1:10,000
End up with 1 mL of liquid
divide into 4 tubes, 200 uL to each
add 200 uL of liquid (mouthwash) to each tube
wait... 10 min...
plate w/ 100 uL each
wait 1 day.
yeeehawww
WHAT UP LAB GROUP
welcome to our super sweet blog, where we will post our lab sections as we finish them so that others can copy and paste them into word and then improve on their greatness. mouthwash rules!
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